- At the very beginning of his labors as minister of Edinburgh, John Knox had the misfortune to lose his much-loved and helpful young wife. She left two sons, one of whom, Nathanael, died at Cambridge in 1580; the other, Eleazer, became vicar of Clacton Magna in the archdeaconry of Colchester and died in 1591. In 1564 Knox made a second marriage, which was greatly talked of at the time because the bride was remotely connected with the royal family. Even more so, the marriage receive much attention because John Knox was 50, while she was a maiden of seventeen! The young lady was Margaret Stewart, daughter of Andrew, Lord Stewart of Ochiltree. She bore Knox three daughters, of whom the youngest, Elizabeth, became the wife of the famous John Welsh, minister of Ayr.